Extracting Capsaicin From Pepper X to Make the World's Hottest Sauce

187,423
0
Published 2024-01-27
In late 2023, Ed Currie's Pepper-X was crowned the new hottest pepper in the world, with an astounding Scoville heat rating of 2,693,000 (compared to the Carolina Reaper's previous 1,600,000-2,200,000). So logically, my first instinct was to use chemistry to make it over five times hotter!

UPDATE: the capsaicin giveaway has officially ended, there are no more samples available!

Want to support LabCoatz? Of course you do! Feel free to join my Patreon here:
www.patreon.com/LabCoatz
www.paypal.com/paypalme/labcoatz

Credit goes to ‪@FirstWeFeast‬ for some of the pepper-related video content used:    • Pepper X: Sean Evans, Chili Klaus & S...  

Some footage from ‪@NileRed‬ and ‪@ShawnWoodsMousetrapMonday‬ was also used:
   • Turning plastic gloves into hot sauce  
   • Does The World's Spiciest Pure Capsai...  

Also, a shoutout goes to ‪@ChaseTheHeat‬ for actually testing the Scoville rating of the Hot Ones Xperience sauce. As it turns out, it might be way less spicy than I thought: only about 61,000 SHU! Its possible my bottle was a bit hotter, since it felt worse than the sauce I made exclusively from Carolina Reapers and ghost peppers, but I really can't be sure. It does kind of check out though: if the sauce really was over 2,000,000 Scoville, I should've gotten way more capsaicin out...up to FORTY TIMES MORE! Several GRAMS of the hot stuff. Regardless, the capsaicin I did obtain still should've been fairly pure and from Pepper X.

And be sure to check out ‪@TechIngredients‬ video on making a super-hot sauce from pepper extract! He didn't purify his capsaicin with chromatography or liquid-liquid extraction, but he definitely came away with a more edible (and similarly spicy) sauce:
   • We made the Spiciest Hot Sauce Ever!?  

WARNING: although everything I do here is legal, some of the content shown on LabCoatz is dangerous and should not be repeated! This procedure was fairly tame, but in general, it's not a very good idea to taste or eat things that have been in contact with chemicals or labware. I took all of the necessary precautions to remain safe!

Also, to avoid legal trouble, I feel like I should elaborate on what I said towards the end of this video about "cooking a legal version of meth" in a future video: the "legal meth" is a safe, off-the-shelf cold medicine called propylhexedrine, and although its molecule is shaped almost exactly like methamphetamine's molecule, it is not addictive and is generally not used recreationally due to it's unpleasant side effects. It can be bought at almost any American store as "Benzedrex" without even needing an ID, so me making a small amount for demonstration purposes should be completely fine.

0:00 Introduction
0:34 Project backstory
1:44 Prepping the sauce
2:45 Ethanol extraction
4:34 Steam distillation
5:59 Column chromatography
9:06 Acid/base extraction
9:55 The tasting lineup
11:32 Taste test: Louisiana Hot Sauce (2000 Scoville)
12:58 Taste test: homemade Reaper sauce (~1,000,000 Scoville)
14:11 Taste test: Last Dab Xperience (2,000,000+ Scoville...or 61,000? Idk)
16:02 Taste test: Habanero pepper (100,000-300,000 Scoville)
18:37 Taste test: PURIFIED CAPSAICIN (15,000,000+ Scoville)
22:55 DO YOU WANT SOME?
23:27 Conclusion

All Comments (21)
  • @LabCoatz_Science
    Alright, I've heard the news: according to Johnny on "Chase The Heat" about a day ago, the Xperience sauce barely scored over 60,000 Scovilles according to lab tests. Not sure what's up with that. My bottle was definitely hotter than the sauce I made from Reapers and ghost peppers, which I assume is hotter than at least 500,000 SHU...maybe Johnny got a bad batch of sauce?? I really don't know. Maybe my peppers were weak! It does kind of check out though: if the sauce really was over 2,000,000 Scoville, I should've gotten way more capsaicin out...like up to FORTY TIMES MORE! Several GRAMS. Regardless, I'm glad to have extract at least some of Ed Currie's precious capsaicin! He can keep Pepper X out of reach for now, but at least I've got something that's hotter!
  • Fun fact - one of the reasons we hot sauce enthusiasts love really, really hot peppers is so they don't need to use extracts to make really hot sauces. It's because, as you learned, they don't taste very pleasant.
  • @Nefville
    You might be surprised to hear this but that exact same Pepper X sauce was just tested and came back at 61,019 SHU. I'm not a chemist but I grow superhot peppers and there are some things that just don't add up about Pepper X and that sauce in particular. If you want a truly world class superhot pepper get a Chocolate Primotalii.
  • Once back in the day, (sometime around 2006) I was working with some kids at a restaurant. One of them started to brag about how they could handle the hottest things period no problem. The next day, I went to the grocery store and purchased some fresh scotch bonnet peppers. The next shift, after dinner rush, I brought the convo back to spicy hot foods. Just as I thought, the kid started his boasting and bragging about his hot tolerance. That is when I busted out the scotch bonnets and offered him one. His eyes went wide but to his credit, he didn't back down. I set the condition that he has to chew it at least 10 times before swallowing. To the kids credit, he did it. Then promptly suffered miserably. Sweat, red face, tears, moaning while rocking back and forth. The works. I didn't tell him about the milk trick so he was guzzling water. Once his ordeal was over. He admitted that he couldn't handle super hot. He never boasted or even brought up spicey hot tolerances, ever again.
  • @CanesTech
    When I was a kid in the late 60's, my Navy Dad claimed a friend of his won a bet (aboard their ship) by drinking a whole bottle of Tabasco Sauce. The Hot Sauce industry has now advanced beyond my wildest dreams. ❤
  • @mikea683
    This stuff is incredible! I worked at a food factory around 15 years ago. We used to have capsaicin on salt and capsaicin oil which we used for the industrial production of sauces. I managed to get a single grain of the salt in my eye while taking a sample for QA and I cried spicy tears for over a week. One guy spilt a bottle of the oil on his lap and had to go to hospital. We had showers in our locker rooms, and he'd been in there to try and clean himself up (in a panic). Later that day when people showered they complained about their feet feeling like they were burning.
  • @john1182
    This is a very similar process to what we currently use to extract morphine/ codeine/ thebaine and oripavin from poppy straw. (especially the chromatography) Ive worked 15 years at a factory that can produce 1/3 of he worlds opioids. This video explained more then almost all of the training at the average factory workers revived. the exception was the labs and R&D staff. Well done.
  • @seang2012
    The REAL science happens in the bathroom when that spice works through Zach's biology.
  • @brucecook502
    There is a very good and simple explanation for why that couple little bites from the red habanero was way spicier than that Xperience sauce. That's because the red habanero can get up to 500,000 shu, but the experience sauce was recently found to only test at 61k shu. Whoever put on the label that the Xperience sauce is 91% of a pepper the averages almost 2.7 million shu was fos...
  • Major bonus points for running a column on this. I really wish it was more frequent in YouTube chemistry videos.
  • @Fuzz_Aldrin88
    Its worth mentioning that capsaicin isnt the only culprit responsible for a pepper's spice. There are quite a few other capsaicinoids that contribute. This is why different types of spiciness exist. Like, a jabanero isnt the same 'kind of heat' that you might get off of a pepper with a similar scoville rating, because different ratios of different capsaicinoids change the heat level and type of burn drastically. Not sure if your process was selectively extracting raw capsaicin, or if it was designed to pull all the lesser capsaicinoids out as well.
  • @StormBurnX
    Completely unrelated to the actual video content itself - I LOVE seeing the board games in the background. Ticket to Ride is such a wild bunch of fun with the right people.
  • @andrews.4780
    The column chromatography made this a next level extraction! This was definitely the coolest capsaicin video I’ve seen 🔥👏🏼
  • @Blaaake
    You sir have some balls. Sub earned. Looking forward to more videos.
  • Doesn't the vinegar in the sauce react with the aluminiumfoil during drying? A foodgrade silicone mat might be better?
  • @CajunReaper95
    I like that Louisiana hot sauce is finally getting to use in these videos!